Journal: BMC Biology

Article Title: Tankyrase inhibition impairs directional migration and invasion of lung cancer cells by affecting microtubule dynamics and polarity signals

doi: 10.1186/s12915-016-0226-9

Figure Lengend Snippet: Centrosome reorientation is retarded by tankyrase I and 2 (TNKS/2) inhibitors in migrating cells. Representative confocal images of centrosome localization in migrating A549 cells. Wound-edge A549 cells, with or without TNKS/2 inhibitors (10 μM), were stimulated with hepatocyte growth factor ( HGF ) (50 ng/mL) for 4 h. Immunofluorescence staining of centrosomes was carried out using anti-γ-tubulin antibody. Centrosome polarization was assessed at the indicated time points. Pie charts display the percentage distribution of distinct angles ( α ) formed by the nuclear-centrosome axis ( red arrows ) and a line perpendicular to the wound ( white arrows ). Centrosomes were considered oriented when α < 30°. A minimum of 136 cells was analyzed for centrosome polarization in three independent experiments. P -values were calculated by the Chi-square test using Graphpad Quickcalcs software. Scale bar, 7 μm. Raw data are shown in Additional file . DMSO dimethyl sulfoxide

Article Snippet: P -values were calculated by the Chi-square test using Graphpad Quickcalcs software.

Techniques: Immunofluorescence, Staining, Software